Article
The 3' untranslated region of human vimentin mRNA interacts with protein complexes containing eEF-1gamma and HAX-1.
Nucleic acids research - 1 Dec 2002
Al-Maghrebi May, Brulé Hervé, Padkina Marina, Allen Carrie, Holmes W Michael, Zehner Zendra E
Abstract excerpt
Previously, we have shown that the vimentin 3' untranslated region (3'UTR) contains a highly conserved region, which is sufficient for the perinuclear localization of a reporter mRNA. This region was shown to specifically bind protein(s) by band shift analyses. UV-cross-linking studies suggest these proteins are 46- and 35-kDa in mass. Here, we have used this sequence as 'bait' to isolate RNA binding proteins...
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