Article
Improved generation of HLA class I/peptide tetramers.
Journal of immunological methods - 20 Dec 2002
Sato Yuriko, Sahara Hiroeki, Tsukahara Tomohide, Kondo Masako, Hirohashi Yoshihiko, Nabeta Yuki, Kawaguchi Satoshi, Ikeda Hideyuki, Torigoe Toshihiko, Ichimiya Shingo, Tamura Yasuaki, Wada Takuro, Yamashita Toshihiko, Goto Masashi, Takasu Hideo, Sato Noriyuki
Abstract excerpt
As a tetrameric major histocompatibility complex (MHC) class I-peptide complex (tetramer) is capable of detecting antigen-specific cytotoxic T lymphocyte (CTL) by flow cytometry, significant information about the generation of in vivo immunity can be obtained. It is, however, difficult to make a soluble wild type of MHC class I heavy chain by the prokaryotic expression system. Therefore, we developed a new method...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.