Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A Novel Gene Expression Control System and Its Use in Stable, High-Titer 293 Cell-Based Adeno-Associated Virus Packaging Cell Lines

18 Nov 2002

Abstract excerpt

Previous attempts to establish 293cell-based stable and high-titer adeno-associated virus (AAV) packaging cell lines were unsuccessful, primarily due to adenovirus E1-activated Rep gene expression, which exerts cytostatic and cytotoxic effects on the host cells. Control of the two large AAV Rep proteins (Rep78/68) was insufficient to eliminate the adverse effects, because of the leaky expression of the two small...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CAR-T cell therapy research
Share to Viral Infectious Diseases and Gene Expression in Insects
Share to Virus-based gene therapy research

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 12438627
DOI: 10.1128/jvi.76.24.13015-13027.2002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A Novel Gene Expression Control System and Its Use in Stable, High-Titer 293 Cell-Based Adeno-Associated Virus Packaging Cell LinesPMID 12438627Read ↗

Inducible HEK293 AAV packaging cell lines expressing Rep proteinsSemantic proximity Read ↗

Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production.Semantic proximity Read ↗

Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfectionSemantic proximity Read ↗

Cell lines inducibly expressing the adeno-associated virus (AAV) rep gene: requirements for productive replication of rep-negative AAV mutants.Semantic proximity Read ↗

Detailed Protocol for the Novel and Scalable Viral Vector Upstream Process for AAV Gene Therapy ManufacturingSemantic proximity Read ↗

An Enhanced System for Construction of Adenoviral Vectors by the Two-Plasmid Rescue MethodSemantic proximity Read ↗

Analysis of adeno-associated virus (AAV) wild-type and mutant Rep proteins for their abilities to negatively regulate AAV p5 and p19 mRNA levels.Semantic proximity Read ↗

Adeno-associated virus type 2 p5 promoter: a rep-regulated DNA switch element functioning in transcription, replication, and site-specific integration.Semantic proximity Read ↗

Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension CultureSemantic proximity Read ↗

Construction of stable packaging cell lines for clinical lentiviral vector productionSemantic proximity Read ↗

A Versatile Adeno-Associated Virus Vector Producer Cell Line Method for Scalable Vector Production of Different SerotypesSemantic proximity Read ↗

A helper-dependent adenovirus vector system: Removal of helper virus by Cre-mediated excision of the viral packaging signalSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.