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Article

Production of combinatorial libraries of fused genes by sequential transposition reactions.

Nucleic acids research - 1 Nov 2002

Naumann Todd A, Goryshin Igor Y, Reznikoff William S

Abstract excerpt

The use of in vivo and in vitro transposition reactions to perform non-combinatorial manipulation of DNAs in molecular biology is widespread. In this work we describe a technique that utilizes two sequential, directed transposition reactions in order to carry out combinatorial DNA manipulations. The methodology relies on the use of two different mutant Tn5 transposase proteins that have different transposon end...

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Identifiers

PMID: 12409478
DOI: 10.1093/nar/gnf118
PMCID: PMC135842

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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