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Article

A transposase strategy for creating libraries of circularly permuted proteins.

Nucleic acids research - 1 May 2012

Mehta Manan M, Liu Shirley, Silberg Jonathan J

Abstract excerpt

A simple approach for creating libraries of circularly permuted proteins is described that is called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the transposase MuA is used to randomly insert a minitransposon that can function as a protein expression vector into a plasmid that contains the open reading frame (ORF) being permuted. A library of vectors that express different permuted variants...

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Identifiers

PMID: 22319214
DOI: 10.1093/nar/gks060
PMCID: PMC3351165

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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