Article
Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis.
Nucleic acids research - 27 Jul 2018
Atkinson Joshua T, Jones Alicia M, Zhou Quan, Silberg Jonathan J
Abstract excerpt
Deep mutational scanning has been used to create high-resolution DNA sequence maps that illustrate the functional consequences of large numbers of point mutations. However, this approach has not yet been applied to libraries of genes created by random circular permutation, an engineering strategy that is used to create open reading frames that express proteins with altered contact order. We describe a new method,...
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