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Article

Screening for recombinant glutathione transferases active with monochlorobimane.

Analytical biochemistry - 1 Oct 2002

Eklund Birgitta I, Edalat Maryam, Stenberg Gun, Mannervik Bengt

Abstract excerpt

A rapid and facile colony assay has been developed for catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferases (GST), expressed in Escherichia coli. The basis of the method is the conjugation of glutathione (GSH) with the fluorogenic substrate monochlorobimane (MCB). This screening method makes it possible to isolate and characterize one recombinant clone that is active...

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PMID: 12381368
DOI: 10.1016/s0003-2697(02)00258-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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