Article
Screening for recombinant glutathione transferases active with monochlorobimane.
Analytical biochemistry - 1 Oct 2002
Eklund Birgitta I, Edalat Maryam, Stenberg Gun, Mannervik Bengt
Abstract excerpt
A rapid and facile colony assay has been developed for catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferases (GST), expressed in Escherichia coli. The basis of the method is the conjugation of glutathione (GSH) with the fluorogenic substrate monochlorobi...
Topics
- Animals
- Cloning, Molecular
- Combinatorial Chemistry Techniques
- DNA, Complementary
- Escherichia coli
- Flow Cytometry
- Genetic Variation
- Glutathione
- Glutathione Transferase
- Humans
- Mass Screening
- Naphthalenes
- Plasmids
- Pyrazoles
- Rats
- Recombinant Proteins
- Scattering, Radiation
- Sensitivity and Specificity