Article
Regulation of the F plasmid traY promoter in Escherichia coli K12 as a function of sequence context.
Journal of molecular biology - 20 Jul 1991
Silverman P M, Wickersham E, Rainwater S, Harris R
Abstract excerpt
TraJ and SfrA are, respectively, plasmid and host (Escherichia coli)-encoded proteins normally required for F plasmid traY promoter function. Beginning with plasmids in which a traY-lacZ fusion gene, designated phi (traY'-'lacZ)hyb, and lacY are expressed from the F plasmid traY promoter, we isolated mutants in which lac gene expression was SfrA or TraJ-independent. A total of 45 of 50 SfrA-independent isolates...
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