Article
Direct analysis of single-nucleotide polymorphism on double-stranded DNA by pyrosequencing.
Biotechnology and applied biochemistry - 1 Apr 2000
Nordström T, Ronaghi M, Forsberg L, de Faire U, Morgenstern R, Nyrén P
Abstract excerpt
Pyrosequencing, a new method for DNA sequencing, is gaining widespread use for many different types of DNA analysis. The method takes advantage of four coupled enzymes in a single tube assay to monitor DNA synthesis in real time using a luminometric detection system. Here, we demonstrate the use of pyrosequencing for direct analysis of single-nucleotide polymorphism on double-stranded PCR product. Pyrosequencing...
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