Article
High sensitivity of detection of TP53 somatic mutations by fluorescence-assisted mismatch analysis.
Genes, chromosomes & cancer - 1 Sept 2002
Tessitore Alessandra, Di Rocco Zorika Christiana, Cannita Katia, Ricevuto Enrico, Toniato Elena, Tosi Mario, Ficorella Corrado, Frati Luigi, Gulino Alberto, Marchetti Paolo, Martinotti Stefano
Abstract excerpt
We analyzed exons 5-9 of the TP53 gene in 41 breast cancers using direct sequencing, PCR-SSCP (single-strand conformation polymorphism), and fluorescence-assisted mismatch analysis (FAMA), to test the level of specificity and sensitivity of each method. A major issue for the correct detection of TP53 somatic mutations in primary tumors is often represented by the large amount of normal DNA, which can cause...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.