Article
Detection of p53 gene mutations: analysis by single-strand conformation polymorphism and Cleavase fragment length polymorphism.
Electrophoresis - 1 Jun 1999
O'Connell C D, Atha D H, Oldenburg M C, Tian J, Siebert M, Handrow R, Grooms K, Heisler L, de Arruda M
Abstract excerpt
We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clones were then used to address the sensitivity of mutation detection using slab-gel single-strand conformation polymorphism (SSCP) and Cleavase fragment length...
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