Article
Ligation of a primer at a mutation: a method to detect low level mutations in DNA.
Mutagenesis - 1 Sept 2002
Kaur Manjit, Zhang Yuzhi, Liu Wei-Hua, Tetradis Sotirios, Price Brendan D, Makrigiorgos G Mike
Abstract excerpt
Detection of low frequency mutations following exposure to mutagens or during the early stages of cancer development is instrumental for risk assessment and molecular diagnosis. We present a sensitive new method to detect trace levels of DNA mutations induced within a large excess of wild-type sequences. The method is based on mutation-induced generation of new restriction enzyme recognition sites. A DNA sequence...
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