Article
Important contribution to catalysis of peptide bond formation by a single ionizing group within the ribosome.
Molecular cell - 1 Aug 2002
Katunin Vladimir I, Muth Gregory W, Strobel Scott A, Wintermeyer Wolfgang, Rodnina Marina V
Abstract excerpt
The catalytic mechanism of peptide bond formation on the ribosome is not known. The crystal structure of 50S ribosomal subunits shows that the catalytic center consists of RNA only and suggests potential catalytic residues. Here we report rapid kinetics of the peptidyl transferase reaction with puromycin at rates up to 50 s(-1). The rate-pH profile of the reaction reveals that protonation of a single ribosomal...
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