Article
Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav.
Biochemistry - 27 Aug 2002
Perez-Villar Juan J, Whitney Gena S, Sitnick Mitchell T, Dunn Robert J, Venkatesan Srividhya, O'Day Kathleen, Schieven Gary L, Lin Tai-An, Kanner Steven B
Abstract excerpt
The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LAT is phosphorylated by protein tyrosine kinases (PTK) and binds to the adaptors Gads and Grb2, as...
Topics
- Adaptor Proteins, Signal Transducing
- Animals
- Binding Sites
- Blotting, Western
- COS Cells
- Carrier Proteins
- Cell Cycle Proteins
- Enzyme-Linked Immunosorbent Assay
- Humans
- Jurkat Cells
- Lymphocyte Activation
- Membrane Proteins
- Mutagenesis, Site-Directed