Article
Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.
Bioconjugate chemistry - 17 Dec 2014
Taskinen Barbara, Zauner Dominik, Lehtonen Soili I, Koskinen Masi, Thomson Chloe, Kähkönen Niklas, Kukkurainen Sampo, Määttä Juha A E, Ihalainen Teemu O, Kulomaa Markku S, Gruber Hermann J, Hytönen Vesa P
Abstract excerpt
Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities...
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