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Article

Engineering soluble monomeric streptavidin with reversible biotin binding capability.

The Journal of biological chemistry - 17 Jun 2005

Wu Sau-Ching, Wong Sui-Lam

Abstract excerpt

Monomeric streptavidin with reversible biotin binding capability has many potential applications. Because a complete biotin binding site in each streptavidin subunit requires the contribution of tryptophan 120 from a neighboring subunit, monomerization of the natural tetrameric streptavidin can generate streptavidin with reduced biotin binding affinity. Three residues, valine 55, threonine 76, and valine 125,...

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Identifiers

PMID: 15840576
DOI: 10.1074/jbc.M501733200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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