Article
Downregulation of MMP-9 in ERK-mutated stable transfectants inhibits glioma invasion in vitro.
Oncogene - 15 Aug 2002
Lakka Sajani S, Jasti Sushma L, Gondi Christopher, Boyd Douglas, Chandrasekar Nirmala, Dinh Dzung H, Olivero William C, Gujrati Meena, Rao Jasti S
Abstract excerpt
We previously showed that enhanced expression of MMP-9, an endopeptidase that digests basement-membrane type IV collagen, is related to tumor progression in vitro and in vivo; antisense-MMP-9 stably transfected clones were less invasive than untransfected parental cells and did not form tumors in nude mice. In this study, we examined the role of ERK-1 in the regulation of MMP-9 production and the invasive...
Topics
- Blotting, Northern
- Blotting, Western
- Brain Neoplasms
- Cell Division
- Collagen
- Down-Regulation
- Drug Combinations
- Electrophoretic Mobility Shift Assay
- Gelatin
- Glioma
- Humans
- Laminin
- Matrix Metalloproteinase 9