Article
Large-scale monocyte enrichment coupled with a closed culture system for the generation of human dendritic cells.
Journal of immunological methods - 15 Sept 2002
Pullarkat Vinod, Lau Roy, Lee Sun-Min, Bender James G, Weber Jeffrey S
Abstract excerpt
Conventional methods for generating monocyte-derived dendritic cells (DC) for clinical trials utilize the property of plastic adherence to select monocytes from leukapheresis samples. This method is labor-intensive and has the potential for contamination at various steps. We evaluated a large-scale monocyte enrichment procedure using a cell selector (Isolex 300i(R)) followed by culture in a sterile bag system...
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