Comparing methods for ex vivo characterization of human monocyte phenotypes and in vitro responses.

doi:10.1016/j.imbio.2015.07.014

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Article

Comparing methods for ex vivo characterization of human monocyte phenotypes and in vitro responses.

Immunobiology - 1 Dec 2015

Johnston Lisa, Harding Scott A, La Flamme Anne Camille

Abstract excerpt

Monocytes are key innate effector cells and their phenotype and function may be a useful biomarker of disease state or therapeutic response. However, for such an assay to be clinically feasible it needs to be simple and reproducible, which this study aimed to address. Peripheral blood mononuclear cells (PBMC)(2) isolated from whole blood using Histopaque-1077 or cell preparation tubes (CPT) showed no difference...

Topics

Adult
Antigens, Surface
Biomarkers
Cell Separation
Cluster Analysis
Cytokines
Female
Flow Cytometry
Gene Expression Profiling
Humans
Immunophenotyping
Leukocytes, Mononuclear
Lipopolysaccharide Receptors
Male
Middle Aged
Monocytes
Phenotype
Young Adult

Source

Open in PubMed

Identifiers

PMID: 26256247
DOI: 10.1016/j.imbio.2015.07.014

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