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Article

Mutational analysis of polynucleotide phosphorylase from Escherichia coli.

Journal of molecular biology - 16 Aug 2002

Jarrige Anne, Bréchemier-Baey Dominique, Mathy Nathalie, Duché Ophélie, Portier Claude

Abstract excerpt

Polynucleotide phosphorylase (PNPase), a homotrimeric exoribonuclease present in bacteria, is involved in mRNA degradation. In Escherichia coli, expression of this enzyme is autocontrolled at the translational level. We introduced about 30 mutations in the pnp gene by site-directed mutagenesis, most of them in phylogenetically conserved residues, and determined their effects on the three catalytic activities of...

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Identifiers

PMID: 12162954
DOI: 10.1016/s0022-2836(02)00645-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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