Isolation and characterization of a new temperature-sensitive polynucleotide phosphorylase mutation in Escherichia coli K-12.

doi:10.1016/0300-9084(90)90193-k

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Article

Isolation and characterization of a new temperature-sensitive polynucleotide phosphorylase mutation in Escherichia coli K-12.

Biochimie - 1 Nov 1990

Yancey S D, Kushner S R

Abstract excerpt

Polynucleotide phosphorylase (PNPase) has been studied in detail since its discovery in 1955 [1]. In an attempt to determine what role, if any, it has in mRNA decay in Escherichia coli, we have isolated and characterized a temperature-sensitive mutation, pnp-200, in the pnp gene. In vitro phospho...

Topics

Escherichia coli
Genes, Bacterial
Genotype
Hot Temperature
Kinetics
Mutation
Plasmids
Polyribonucleotide Nucleotidyltransferase
Restriction Mapping
Thermodynamics

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