Article
PNPase autocontrols its expression by degrading a double-stranded structure in the pnp mRNA leader.
The EMBO journal - 3 Dec 2001
Jarrige A C, Mathy N, Portier C
Abstract excerpt
Polynucleotide phosphorylase synthesis is autocontrolled at a post-transcriptional level in an RNase III-dependent mechanism. RNase III cleaves a long stem-loop in the pnp leader, which triggers pnp mRNA instability, resulting in a decrease in the synthesis of polynucleotide phosphorylase. The staggered cleavage by RNase III removes the upper part of the stem-loop structure, creating a duplex with a short 3'...
Topics
- 5' Untranslated Regions
- Base Sequence
- Binding Sites
- Endoribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Enzymologic
- Models, Biological
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids