Article
NinR- and red-mediated phage-prophage marker rescue recombination in Escherichia coli: recovery of a nonhomologous immlambda DNA segment by infecting lambdaimm434 phages.
Genetics - 1 Aug 2005
Hayes Sidney, Asai Kengo, Chu Audrey M, Hayes Connie
Abstract excerpt
We examined the requirement of lambda recombination functions for marker rescue of cryptic prophage genes within the Escherichia coli chromosome. We infected lysogenic host cells with lambdaimm434 phages and selected for recombinant immlambda phages that had exchanged the imm434 region of the infecting phage for the heterologous 2.6-kb immlambda region from the prophage. Phage-encoded activity, provided by either...
Topics
- Bacteriophage lambda
- Chromosomes, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Genes, Viral
- Mutation
- Prophages
- Rec A Recombinases
- Recombination, Genetic
- Sequence Deletion
- Temperature