Tools for characterization of Escherichia coli genes of unknown function.

doi:10.1128/JB.184.16.4573-4581.2002

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Article

Tools for characterization of Escherichia coli genes of unknown function.

Journal of bacteriology - 1 Aug 2002

Merlin Christophe, McAteer Sean, Masters Millicent

Abstract excerpt

Despite the power of sequencing and of emerging high-throughput technologies to collect data rapidly, the definitive functional characterization of unknown genes still requires biochemical and genetic analysis in case-by-case studies. This often involves the deletion of target genes and phenotypic characterization of the deletants. We describe here modifications of an existing deletion method which facilitates...

Topics

Alleles
Escherichia coli
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Bacterial
Mutagenesis, Insertional
Phenotype
Plasmids

Source

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Identifiers

PMID: 12142427
DOI: 10.1128/JB.184.16.4573-4581.2002
PMCID: PMC135234

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