Article
Tools for characterization of Escherichia coli genes of unknown function.
Journal of bacteriology - 1 Aug 2002
Merlin Christophe, McAteer Sean, Masters Millicent
Abstract excerpt
Despite the power of sequencing and of emerging high-throughput technologies to collect data rapidly, the definitive functional characterization of unknown genes still requires biochemical and genetic analysis in case-by-case studies. This often involves the deletion of target genes and phenotypic characterization of the deletants. We describe here modifications of an existing deletion method which facilitates...
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