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Article

Scanning the Escherichia coli chromosome by random transposon mutagenesis and multiple phenotypic screening.

Research in microbiology - 1 Oct 2004

Serina Stefania, Nozza Francesca, Nicastro Giovanna, Faggioni Federico, Mottl Harald, Dehò Gianni, Polissi Alessandra

Abstract excerpt

Analysis of the complete DNA sequences of many microbial genomes available reveals a fair number of putative ORFs without an identified function. A systematic scan of the Escherichia coli chromosome was achieved by random transposition with a newly developed Tn5 minitransposon derivative carrying the arabinose-inducible araP(BAD) promoter oriented outward at one end (Tn5-araP(BAD)). The transposon insertion...

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Identifiers

PMID: 15380559
DOI: 10.1016/j.resmic.2004.05.006

Genes

BAD

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Source receipt: pubmed, retrieved 7 Sept 2026.

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