Article
Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33 degrees C in rich media.
Journal of bacteriology - 1 Jan 1991
Karow M, Fayet O, Cegielska A, Ziegelhoffer T, Georgopoulos C
Abstract excerpt
We have identified and studied the htrB gene of Escherichia coli. Insertional inactivation of the htrB gene leads to bacterial death at temperatures above 33 degrees C. The mutant bacterial phenotype at nonpermissive temperatures includes an arrest of cell division followed by the formation of bu...
Topics
- Cloning, Molecular
- Coliphages
- Culture Media
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Genomic Library
- Genotype
- Kinetics
- Plasmids
- Restriction Mapping
- Temperature