SNP genotyping on pooled DNAs: comparison of genotyping technologies and a semi automated method for data storage and analysis.

doi:10.1093/nar/gnf070

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Article

SNP genotyping on pooled DNAs: comparison of genotyping technologies and a semi automated method for data storage and analysis.

Nucleic acids research - 1 Aug 2002

Le Hellard Stéphanie, Ballereau Stéphane J, Visscher Peter M, Torrance Helen S, Pinson Jeni, Morris Stewart W, Thomson Marian L, Semple Colin A M, Muir Walter J, Blackwood Douglas H R, Porteous David J, Evans Kathryn L

Abstract excerpt

We have compared the accuracy, efficiency and robustness of three methods of genotyping single nucleotide polymorphisms on pooled DNAs. We conclude that (i) the frequencies of the two alleles in pools should be corrected with a factor for unequal allelic amplification, which should be estimated from the mean ratio of a set of heterozygotes (k); (ii) the repeatability of an assay is more important than pinpoint...

Topics

Automation
Base Sequence
Biotechnology
Chi-Square Distribution
Chromatography, High Pressure Liquid
Databases, Nucleic Acid
Gene Amplification
Gene Frequency
Genetic Markers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genotype

Humans

Information Storage and Retrieval

Mass Spectrometry

Polymorphism, Single Nucleotide

Reproducibility of Results

Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 12140336
DOI: 10.1093/nar/gnf070
PMCID: PMC137092

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