Article
Polypurine tract formation by Ty1 RNase H.
The Journal of biological chemistry - 14 Dec 2001
Wilhelm M, Uzun O, Mules E H, Gabriel A, Wilhelm F X
Abstract excerpt
To better understand the mechanism by which Ty1 RNase H creates the polypurine tract (PPT) primer, we have demonstrated the polymerase-dependent hydrolytic activity of Ty1 reverse transcriptase (RT) during minus-strand synthesis. Using RNase H and polymerase mutants of the recombinant Ty1 RT protein, we show that the two domains of Ty1 RT can act independently of one another. Our results indicate that RNA/DNA...
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