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Mutating a Conserved Motif of the HIV-1 Reverse Transcriptase Palm Subdomain Alters Primer Utilization

1 May 1997

Abstract excerpt

In order to investigate how primer grip residues of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contribute toward the architecture of its palm subdomain and neighboring structural elements, the DNA polymerase and ribonuclease H (RNase H) activities of enzymes bearing aromatic substitutions at Trp229 and Tyr232 of the catalytically-competent p66 subunit were evaluated. Although all mutants...

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PMID: 9153416
DOI: 10.1021/bi963045e

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