Article
Optimizing multi-step B-side charge separation in photosynthetic reaction centers from Rhodobacter capsulatus.
Biochimica et biophysica acta - 1 Feb 2016
Faries Kaitlyn M, Kressel Lucas L, Dylla Nicholas P, Wander Marc J, Hanson Deborah K, Holten Dewey, Laible Philip D, Kirmaier Christine
Abstract excerpt
Using high-throughput methods for mutagenesis, protein isolation and charge-separation functionality, we have assayed 40 Rhodobacter capsulatus reaction center (RC) mutants for their P(+)QB(-) yield (P is a dimer of bacteriochlorophylls and Q is a ubiquinone) as produced using the normally inactive B-side cofactors BB and HB (where B is a bacteriochlorophyll and H is a bacteriopheophytin). Two sets of mutants...
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