Article
Voltage dependence of proton pumping by bacteriorhodopsin mutants with altered lifetime of the M intermediate.
PloS one - 1 Jan 2013
Geibel Sven, Lörinczi Éva, Bamberg Ernst, Friedrich Thomas
Abstract excerpt
The light-driven proton pump bacteriorhodopsin (BR) from Halobacterium salinarum is tightly regulated by the [H(+)] gradient and transmembrane potential. BR exhibits optoelectric properties, since spectral changes during the photocycle are kinetically controlled by voltage, which predestines BR for optical storage or processing devices. BR mutants with prolonged lifetime of the blue-shifted M intermediate would...
Topics
- Animals
- Bacteriorhodopsins
- Models, Molecular
- Mutation
- Patch-Clamp Techniques
- Proton Pumps
- Xenopus