Article
In Situ, Protein-Mediated Generation of a Photochemically Active Chlorophyll Analogue in a Mutant Bacterial Photosynthetic Reaction Center.
Biochemistry - 27 Apr 2021
Magdaong Nikki Cecil M, Buhrmaster James C, Faries Kaitlyn M, Liu Haijun, Tira Gregory A, Lindsey Jonathan S, Hanson Deborah K, Holten Dewey, Laible Philip D, Kirmaier Christine
Abstract excerpt
All possible natural amino acids have been substituted for the native LeuL185 positioned near the B-side bacteriopheophytin (HB) in the bacterial reaction center (RC) from Rhodobacter sphaeroides. Additional mutations that enhance electron transfer to the normally inactive B-side cofactors are present. Approximately half of the isolated RCs with Glu at L185 contain a magnesium chlorin (CB) in place of HB. The...
Topics
- Rhodobacter sphaeroides
- Photosynthetic Reaction Center Complex Proteins
- Chlorophyll
- Bacterial Proteins
- Pheophytins
- Mutation
- Porphyrins
- Bacteriochlorophyll A
- Oxidation-Reduction