Article
Late events in the photocycle of bacteriorhodopsin mutant L93A.
Biophysical journal - 1 Jun 2003
Tóth-Boconádi R, Keszthelyi L, Stoeckenius W
Abstract excerpt
In the photocycle of bacteriorhodopsin (bR) from Halobacterium salinarum mutant L93A, the O-intermediate accumulates and the cycling time is increased approximately 200 times. Nevertheless, under continuous illumination, the protein pumps protons at near wild-type rates. We excited the mutant L93A in purple membrane with single or triple laser flashes and quasicontinuous illumination, (i.e., light for a few...
Topics
- Adaptation, Physiological
- Bacteriorhodopsins
- Cells, Cultured
- Darkness
- Dose-Response Relationship, Radiation
- Halobacterium salinarum
- Lasers
- Membrane Potentials
- Mutation
- Periodicity
- Photic Stimulation
- Photobiology
- Protons
- Purple Membrane