Article
Unveiling the critical role of K+ for xanthorhodopsin expression in E. coli.
Journal of photochemistry and photobiology. B, Biology - 1 Sept 2024
Hour Chenda, Chuon Kimleng, Song Myung-Chul, Shim Jin-Gon, Cho Shin-Gyu, Kang Kun-Wook, Kim Ji-Hyun, Jung Kwang-Hwan
Abstract excerpt
Xanthorhodopsin (XR), a retinal-binding 7-transmembrane protein isolated from the eubacterium Salinibacter ruber, utilizes two chromophores (retinal and salinixanthin (SAL)) as an outward proton pump and energy-donating carotenoid. However, research on XR has been impeded owing to limitations in achieving heterogeneous expression of stable forms and high production levels of both wild-type and mutants. We...
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