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Article

Improving a circularly permuted TEM-1 beta-lactamase by directed evolution.

Protein engineering - 1 Jun 2002

Osuna Joel, Pérez-Blancas Alejandra, Soberón Xavier

Abstract excerpt

Circular permutation of proteins is a powerful technique to explore the importance of the polypeptide secondary structure order for attaining the final three-dimensional structure. Here, we designed a circular permutation of the TEM beta-lactamase in order to produce a new domain-forming amino acid arrangement in the polypeptide sequence. Closing the normal N- and C-termini with the connecting peptide GGS and...

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Source

Identifiers

PMID: 12082164
DOI: 10.1093/protein/15.6.463

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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