Article
Tethered function assays using 3' untranslated regions.
Methods (San Diego, Calif.) - 1 Feb 2002
Coller Jeffery, Wickens Marvin
Abstract excerpt
Proteins that regulate mRNA metabolism are often bipartite: an RNA binding activity confers substrate specificity, and a "functional" domain elicits the biological outcome. In some cases, these two activities reside on different polypeptides that form a complex on the mRNA. Regardless, experimental separation of RNA binding from function facilitates analysis of both properties, liberating each from the...
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