Article
Differential haplotype amplification leads to misgenotyping of heterozygote as homozygote when using single nucleotide mismatch primer.
Electrophoresis - 1 Dec 2012
De Sarkar Navonil, Majumder Mousumi, Roy Bidyut
Abstract excerpt
Mismatches at the 3'end of /or within a primer are reported to affect the efficiency of PCR and cause allele drop. Here, we report preferential amplification of one haplotype and misgenotyping, when double heterozygotes at NAT1 (rs1057126 and rs15561) were genotyped by sequencing and PCR-RFLP methods using mismatch reverse primers located next to the target SNP. Detailed study revealed highest (100%) and lowest...
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