Article
Functional analysis of yeast snoRNA and snRNA 3'-end formation mediated by uncoupling of cleavage and polyadenylation.
Molecular and cellular biology - 1 Mar 2002
Morlando Mariangela, Greco Paolo, Dichtl Bernhard, Fatica Alessandro, Keller Walter, Bozzoni Irene
Abstract excerpt
Many nuclear and nucleolar small RNAs are accumulated as nonpolyadenylated species and require 3'-end processing for maturation. Here, we show that several genes coding for box C/D and H/ACA snoRNAs and for the U5 and U2 snRNAs contain sequences in their 3' portions which direct cleavage of primary transcripts without being polyadenylated. Genetic analysis of yeasts with mutations in different components of the...
Topics
- Base Sequence
- Chromosomes, Fungal
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Polyadenylation
- RNA 3' End Processing
- RNA Precursors
- RNA, Fungal
- RNA, Small Nuclear
- RNA, Small Nucleolar