Article
Genetic rescue of Leishmania deficiency in porphyrin biosynthesis creates mutants suitable for analysis of cellular events in uroporphyria and for photodynamic therapy.
The Journal of biological chemistry - 26 Apr 2002
Sah Jerome Franklin, Ito Hiroya, Kolli Bala Krishna, Peterson Daniel A, Sassa Shigeru, Chang Kwang-Poo
Abstract excerpt
Leishmania was found deficient in at least five and most likely seven of the eight enzymes in the heme biosynthesis pathway, accounting for their growth requirement for heme compounds. The xenotransfection of this trypanosomatid protozoan led to their expression of the mammalian genes encoding delta-aminolevulinate (ALA) dehydratase and porphobilinogen deaminase, the second and the third enzymes of the pathway,...
Topics
- Animals
- Animals, Genetically Modified
- Base Sequence
- DNA Primers
- Hydroxymethylbilane Synthase
- Leishmania
- Microscopy, Fluorescence
- Mutation
- Photochemotherapy
- Porphobilinogen Synthase
- Porphyrias
- Porphyrins
- Transfection