Article
PTR1-dependent synthesis of tetrahydrobiopterin contributes to oxidant susceptibility in the trypanosomatid protozoan parasite Leishmania major.
Current genetics - 1 Jun 2009
Nare Bakela, Garraway Levi A, Vickers Tim J, Beverley Stephen M
Abstract excerpt
Leishmania must survive oxidative stress, but lack many classical antioxidant enzymes and rely heavily on trypanothione-dependent pathways. We used forward genetic screens to recover loci mediating oxidant resistance via overexpression in Leishmania major, which identified pteridine reductase 1 (PTR1). Comparisons of isogenic lines showed ptr1 (-) null mutants were 18-fold more sensitive to H(2)O(2) than...
Topics
- Animals
- Biopterins
- Drug Resistance
- Folic Acid
- Hydrogen Peroxide
- Leishmania major
- Methylnitronitrosoguanidine
- Molsidomine
- Multienzyme Complexes
- Mutation
- Nitric Oxide Donors
- Nitroso Compounds
- Oxidants
- Oxidoreductases