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Article

mRNA secondary structure can greatly affect production of recombinant phospholipase A(2) toxins in bacteria.

Toxicon : official journal of the International Society on Toxinology - 1 May 2002

Ivanovski G, Gubensek F, Pungercar J

Abstract excerpt

The neurotoxic activity of ammodytoxin A (AtxA), a phospholipase A(2) from Vipera ammodytes ammodytes venom, has been investigated by protein engineering. With the aim of obtaining AtxA as a non-fused protein in the bacterial cytoplasm and avoiding problems with incomplete cleavage in vivo of the initial Met preceding the first residue (Ser1), a double mutant (S1A/E4Q) was prepared and expressed in Escherichia...

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PMID: 11821126
DOI: 10.1016/s0041-0101(01)00250-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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