Article
Direct molecular cloning and expression of two distinct abrin A-chains.
The Journal of biological chemistry - 15 Apr 1991
Evensen G, Mathiesen A, Sundan A
Abstract excerpt
The protein toxin abrin, which possesses an N-glycosylase activity toward eukaryotic 28 S rRNA, may have a potential in the deliberate eradication of certain cells. Here we report, by polymerase chain reaction technique, the isolation of genomic DNA sequences encoding two distinct abrin A-chains. A third sequence which encoded a part of a third type of A-chain was also isolated. The deduced amino acid sequences...
Topics
- Abrin
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Gene Expression
- Genetic Variation
- Macromolecular Substances
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- RNA, Messenger