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Article

Increasing the thermal stability of an oligomeric protein, beta-glucuronidase.

Journal of molecular biology - 18 Jan 2002

Flores Humberto, Ellington Andrew D

Abstract excerpt

The reporter enzyme beta-glucuronidase was mutagenized and evolved for thermostability. After four cycles of screening the best variant was more active than the wild-type enzyme, and retained function at 70 degrees C, whereas the wild-type enzyme lost function at 65 degrees C. Variants derived fr...

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Source

Identifiers

PMID: 11786015
DOI: 10.1006/jmbi.2001.5223

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-11786015-2026-09-02.1. Manifest publication-graph-cohort-2026-09-02.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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