Article
A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons.
The Journal of cell biology - 7 Jan 2002
Gu Wei, Pan Feng, Zhang Honglai, Bassell Gary J, Singer Robert H
Abstract excerpt
The localization of beta-actin mRNA to the leading lamellae of chicken fibroblasts and neurite growth cones of developing neurons requires a 54-nt localization signal (the zipcode) within the 3' untranslated region. In this study we have identified and isolated five proteins binding to the zipcode. One of these we previously identified as zipcode binding protein (ZBP)1, a 4-KH domain protein. A second is now...
Topics
- Actins
- Amino Acid Sequence
- Animals
- Blotting, Western
- Brain
- Chick Embryo
- Chickens
- Chromatography, Affinity
- Cloning, Molecular
- Cytoplasm
- DNA, Complementary
- Electrophoretic Mobility Shift Assay
- Fibroblasts
- Gene Expression Regulation, Developmental