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Study on immortalization of Mongolian sheep fibroblast cells

7 Feb 2025

Abstract excerpt

This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF. Human (hTERT) and sheep (sTERT) telomerase reverse transcriptase vectors were...

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PMID: 39918276
DOI: 10.1080/10495398.2025.2459915

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