Article
Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression.
Protein engineering - 1 Nov 2001
Sroga G E, Dordick J S
Abstract excerpt
Concomitant activity improvement of an evolved enzyme toward two very different ester substrates was achieved when a unique combination of functional periplasmic enzyme expression in Escherichia coli, random mutagenesis, DNA shuffling and cell-based kinetic screenings was applied. Specifically, we focused on the conversion of subtilisin E into an enzyme with broader esterase activity as opposed to its native...
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