Article
Identification of residues critical for metallo-beta-lactamase function by codon randomization and selection.
Protein science : a publication of the Protein Society - 1 Dec 2001
Materon I C, Palzkill T
Abstract excerpt
IMP-1 beta-lactamase is a zinc metallo-enzyme encoded by the transferable bla(IMP-1) gene, which confers resistance to virtually all beta-lactam antibiotics including carbapenems. To understand how IMP-1 recognizes and hydrolyzes beta-lactam antibiotics it is important to determine which amino acid residues are critical for catalysis and which residues control substrate specificity. We randomized 27 individual...
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