Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Establishment of a sequence-based typing system for BoLA-DRB3 exon 2.

Tissue antigens - 1 Jul 2003

Miltiadou D, Law A S, Russell G C

Abstract excerpt

A rapid, high-resolution sequence-based typing (SBT) system for BoLA-DRB3 exon 2 was developed. Amplification of the entire exon was achieved by a fully nested PCR with locus-specific primers and sequencing was performed directly on the PCR product. Heterozygous sequence data were obtained by automated sequence analysis of both alleles. Forward and reverse sequence data were assembled to improve identification of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cattle
Share to Consensus Sequence
Share to Exons

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Heterozygote

Share to Histocompatibility Antigens Class II

Share to Polymorphism, Restriction Fragment Length

Share to Sensitivity and Specificity

Share to Sequence Deletion

Source

Identifiers

PMID: 12859595
DOI: 10.1034/j.1399-0039.2003.00080.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Establishment of a sequence-based typing system for BoLA-DRB3 exon 2.PMID 12859595Read ↗

A rapid and robust sequence-based genotyping method for BoLA-DRB3 alleles in large numbers of heterozygous cattle.Semantic proximity Read ↗

Identification of new cattle BoLA-DRB3 alleles by sequence-based typing.Semantic proximity Read ↗

Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP.Semantic proximity Read ↗

Short communication: Establishment of a new polymerase chain reaction-sequence-based typing method for genotyping cattle major histocompatibility complex class II DRB3.Semantic proximity Read ↗

Identification of BoLA-DRB3.2 alleles in Korean native cattle (Hanwoo) and Holstein populations using a next generation sequencer.Semantic proximity Read ↗

Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles.Semantic proximity Read ↗

Strong association between polymorphisms in an intronic microsatellite and in the coding sequence of the BoLA-DRB3 gene: implications for microsatellite stability and PCR-based DRB3 typing.Semantic proximity Read ↗

Establishment of a sequence-based typing system for BoLA-DQA1 exon 2.Semantic proximity Read ↗

Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing.Semantic proximity Read ↗

Denaturing gradient gel electrophoresis: a rapid method for differentiating BoLA-DRB3 alleles.Semantic proximity Read ↗

Multi-primer target PCR for rapid identification of bovine DRB3 alleles.Semantic proximity Read ↗

A new method for typing bovine major histocompatibility complex class II DRB3 alleles by combining two established PCR sequence-based techniques.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.