Article
The looped domain organization of the nucleoid in histone-like protein defective Escherichia coli strains.
Biochimie - 1 Sept 2001
Brunetti R, Prosseda G, Beghetto E, Colonna B, Micheli G
Abstract excerpt
We have investigated the major Escherichia coli histone-like proteins (H-NS, HU, FIS, and IHF) as putative factors involved in the maintenance of the overall DNA looped arrangement of the bacterial nucleoid. The long-range architecture of the chromosome has been studied by means of an assay based on in vivo genomic fragmentation mediated by endogenous DNA gyrase in the presence of oxolinic acid. The fragmentation...
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