Article
A mutated cathepsin-D devoid of its catalytic activity stimulates the growth of cancer cells.
Oncogene - 18 Oct 2001
Glondu M, Coopman P, Laurent-Matha V, Garcia M, Rochefort H, Liaudet-Coopman E
Abstract excerpt
Cathepsin-D, a lysosomal aspartyl proteinase, is highly secreted by breast cancer cells and its over-expression by transfection stimulates cancer cell proliferation. The mechanism by which this protease affects proliferation remains, however, unknown. In order to determine whether proteolytic activity is necessary, we abolished its enzymatic activity using site-directed mutagenesis followed by stable transfection...
Topics
- Animals
- Antibodies
- Breast Neoplasms
- Catalysis
- Cathepsin D
- Cell Division
- Enzyme Precursors
- Female
- Kinetics
- Mice
- Mice, Nude
- Mitogens